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- December 6, 2014. Effect of Substrate Concentration on Enzyme Kinetics (Theory (http://amrita.vlab.co.in/%3Fsub%3D3%26brch%3D64%26s im%3D1090%26cnt%3D1) When we plot a
**graph**with substrate concentration on the X axis and... The necessary terms in this reaction are [S], V0,**Vmax**, and Km (Michaelis constant). - December 12, 2014. Enzyme Biochemistry Practice Problem Solutions (http://dwb4.unl.edu/Chem/CHEM869K/CHEM869KLinks/esg -www.mit.edu/esgbio/eb/solutions.html) Draw a new
**graph**on the same**graph**that you did the first**graph**on... The**graphs**below show two ways to estimate KM and**Vmax**from the above data: OR. - December 17, 2014. The Effect of Substrate Concentration on Enzyme Activity (http://www.ucl.ac.uk/~ucbcdab/enzass/substrate.htm) Skip the theory and go straight to: How to determine Km and
**Vmax**... be plotted as a**graph**of rate of reaction (v) against concentration of substrate ([S], and will. - November 30, 2014. [CLOSED] ON
**GRAPH**SET LOOKGRAPH VLINE2 SET VAUTO OFF VMIN 100**VMAX**(http://forums.informationbuilders.com/eve/forums/a/ tpc/f/7971057331/m/1917031616) Basically can I set the Axes min and max on the 2nd y axis, the following combination only seems to set it for 1st y axis. ON**GRAPH**SET. - December 1, 2014. A) A
**Graph**Of The Michaelis-Menten Equation Is... | Chegg.com (http://www.chegg.com/homework-help/questions-and-an swers/graph-michaelis-menten-equation-plot-reaction -s-initial-velocity-v0-different-substrate-co-q5767 675) Answer to a) A**graph**of the Michaelis-Menten equation is a plot of a reaction's... First, move the line labeled**Vmax**to a position that represents the maximum. - December 5, 2014. Enzymes: Kinetics - Biochemistry | Fastbleep (http://www.fastbleep.com/biology-notes/40/116/1173) Plot each of your data sets onto your
**graph**, and the points should be linear (or... Three important parameters in a kinetic reaction are**Vmax**, Km and**Vmax**/Km.

Comments about this video:- December 4, 2014. How to calculate Km and
**Vmax**without using a**graph**?!! - Applied (http://www.scienceforums.net/topic/41481-how-to-cal culate-km-and-vmax-without-using-a-graph/) ok, the question is: Calculate Km and**Vmax**for the enzyme reaction with data as below WITHOUT using a graphical method. (10 marks). - December 2, 2014. Is there some software to draw nonlinear
**graphs**from**Vmax**and Km? (http://www.researchgate.net/post/Is_there_some_soft ware_to_draw_nonlinear_graphs_from_Vmax_and_Km) 15 Oct 2013 Is there any software that can draw the nonlinear**graphs**from just Km and**Vmax**values? Because often this is what is given in the publications. - December 13, 2014. How can I calculate Km and
**Vmax**from initial velocity and (https://answers.yahoo.com/question/index%3Fqid%3D20 100304164632AAm5JjR) You can get**Vmax**by making what's called an double reciprocal plot, called a Lineweaver-Burke plot. These are one of the more useful things in. - December 9, 2014. DETERMINE THE Km and
**vmax**OF THE ACIDIC PHOSPHATASE (http://www.slideshare.net/kshehaan/determine-the-km -and-vmax-of-the-acidic-phosphatase) 15 Oct 2009 Table of Contents 1. Title 2. Introduction 3. Principle 4. Materials 5. Procedure 6. Results 7.**Graph**8. Deduction 9. Discussion 1. - December 8, 2014. Enzyme Inhibition - Chemwiki (http://chemwiki.ucdavis.edu/Biological_Chemistry/Ca talysts/Enzymatic_Kinetics/Enzyme_Inhibition) 19 Aug 2014 The
**graph**below shows the path of a reaction both with and without the... changes the apparent value of Km but does not affect the**Vmax**. - December 7, 2014. HOW TO: Calculate Km and
**Vmax**with Excel (http://src.sfasu.edu/~avk/BTC560/HOW%2520TO.htm) This step by step article explains how to calculate Km and**Vmax**using Microsoft Excel. Solver add-in is available as a part of optional component in Excel. - December 10, 2014. Appendix:
**Vmax**and KM Can Be Determined by Double-Reciprocal (http://www.ncbi.nlm.nih.gov/books/NBK22557/) Before the availability of computers, the determination of KM and**Vmax**values required algebraic manipulation of the basic Michaelis-Menten equation. - December 14, 2014. How to Calculate
**Vmax**Lineweaver | The Classroom | Synonym (http://classroom.synonym.com/calculate-vmax-linewea ver-2799.html) The**graph**allows for the calculation of both the**Vmax**and Michaelis-Menten constant (Km) through linear regression of the data points. You will need data from. - December 16, 2014. Measuring KM and
**Vmax**- Campbell (http://web.campbell.edu/faculty/nemecz/323_lect/enz ymes/Measuring%2520KM%2520and%2520Vmax%25202.html) The quantities KM and**Vmax**are experimentally determined and different for each... You can estimate KM and**Vmax**from the**graph**of initial velocity versus [S ]. - December 15, 2014. Determination of
**Vmax**and Km (http://www1.lsbu.ac.uk/water/enztech/determination. html) 6 Aug 2014 Determination of**Vmax**and Km... t = X[S]0/**Vmax**- Km Ln(1 - x... 1/v = Km/**Vmax**[ S]0 + 1/**Vmax**(1.103).**graph**. (b) Eadie-Hofstee plot of v. - December 11, 2014. Structural Biochemistry/Enzyme/Michaelis and Menten Equation (http://en.wikibooks.org/wiki/Structural_Biochemistr y/Enzyme/Michaelis_and_Menten_Equation) Michaelis and Menten
**Graph**. The Michaelis-Menten...**Vmax**is equal to the product of the catalyst rate constant (kcat) and the concentration of the enzyme. - December 3, 2014. Lineweaver-burk woes. - biology biochemistry | Ask MetaFilter (http://ask.metafilter.com/32682/Lineweaverburk-woes) It's been a long time since I've done this, but just looking at the
**graph**you provide,**vMax**is the reciprocal of the y-intercept, and once you have. - March 15, 2015. Graph of Prices for Yamaha V Max in Atlanta, GA (https://motorcyclegraph.com/atlanta/yamaha/v-max) BioChemistryFilter: How do I read the vMax and Km off of a Lineweaver-burk plot?
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- March 18, 2015. Enzyme Kinetics. Getting Km and Vmax from 4 graphs. (http://www.chemicalforums.com/index.php?topic=37884.0) A graph of the double-reciprocal equation is also called a Lineweaver-Burk, 1/Vo vs 1/[S]. The y-intercept is 1/Vmax; the x-intercept is -1/KM; and the slope is KM/Vmax.
- March 19, 2015. Calculating Vmax Km Graph? Askives - Askives - Answers to... (https://www.askives.com/calculating-vmax-km-graph.h tml) The graph below shows the... we can take the double reciprocal and look at the line with no inhibitor to determine ks and vmax. Similar to mixed type inhibition...
- March 20, 2015. HOW TO: Calculate Km and Vmax with Excel (http://src.sfasu.edu/~avk/BTC560/HOW%20TO.htm) Calculating Vmax Km Graph? - Find Questions and Answers at Askives, the first startup that gives you an straight answer
- March 21, 2015. How do i work out the Vmax and the Km from a graph... (https://uk.answers.yahoo.com/question/index?qid=200 70310144647AA8LVPo) Determination of Vmax and Km. Enzyme Technology Determination of V max and K m. It is important to have as thorough knowledge as is possible...
- March 22, 2015. How to work out Km and Vmax from [S] vs 'v' graph? | Yahoo... (https://uk.answers.yahoo.com/question/index?qid=201 21203174404AAEuyas) HOW TO: Calculate Km and Vmax with Excel Summary. This step by step article explains how to calculate Km and Vmax using Microsoft Excel.
- March 23, 2015. GraphPad Curve Fitting Guide - Home - graphpad.com (http://www.graphpad.com/guides/prism/6/curve-fittin g/index.htm?reg_michaelis_menten_enzyme.htm) How do i work out the Vmax and the Km from a graph? Follow. 2 answers. Report Abuse... How to find Vmax and Km using limeweaver burk method?
- March 24, 2015. Vmax Graph - Mobile Resources (http://www.mobileresources.net/mobi/Vmax_Graph/) Does anyoone know how to work out Km and Vmax from [S] vs initial velocity graph?
- March 25, 2015. Equation: Michaelis-Menten model - Home - graphpad.com (http://www.graphpad.com/guides/prism/6/curve-fittin g/reg_michaelis_menten_enzyme.htm) Powder Weight /Velocity Data for the 75gr Hornady Vmax. Graph produced by "RCBS.Load"
- March 26, 2015. Km and Vmax | Student Doctor Network (http://forums.studentdoctor.net/threads/km-and-vmax.653761/) Vmax represents the rate at which an enzyme is completely saturated with substrate. Vmax is often estimated because the value is an asymptote, which cannot be...
- March 27, 2015. Find The Maximum Velocity Vmax Of The Car During... (http://www.chegg.com/homework-help/questions-and-an swers/find-maximum-velocity-vmax-car-ten-second-int erval-depicted-graph-find-maximum-acceleratio-q4252 972) Km and Vmax are related to enzyme kinetics in a biological system. Km is the substrate concentration that is required for the reaction to occur at 1/2 Vmax.
- April 12, 2015. How to Calculate Maximum Velocity | eHow (http://www.ehow.com/how_6142475_calculate-maximum-v elocity.html) Enzyme Kinetics (The Michaelis-Menten Model) It has been shown experimentally that if the amount of an enzyme is kept constant and the concentration of its substrate...
- April 13, 2015. Background - Department of Chemistry [FSU] (http://www.chem.fsu.edu/chemlab/bch4053l/enzymes/in hibition/background.html) The proper techniques for using an oscilloscope are presented. From basic measurements to more advanced techniques.
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- April 18, 2015. Absorbance-time graph and enzyme activity (http://www.chemicalforums.com/index.php?topic=68897.0) Introduction. Kcat is the turnover number the number of substrate molecule each enzyme site converts to product per unit time. If you know the concentration of...
- April 19, 2015. An investigation into the determination of Km and Vmax... (http://www.markedbyteachers.com/university-degree/s ubjects-allied-to-medicine/an-investigation-into-th e-determination-of-km-and-vmax-values-for-alkaline- phosphatase.html) Hi guys! Needing help with a Biochemistry question - 'Express Vmax in terms of concentration change per minute, using your answer from Question 1 to convert...
- April 20, 2015. The Data Below Were Collected For An Enzyme-catalyzed... (http://www.chegg.com/homework-help/questions-and-an swers/data-collected-enzyme-catalyzed-reaction-dete rmine-vmax-km-reaction-q5149332) Chemical Forums > Specialty Chemistry Forums > Biochemistry and Chemical Biology Forum > Absorbance-time graph and enzyme activity
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- April 22, 2015. FAST VP, Unisphere for VMAX, - Upload, Share, and Discover... (http://www.slideshare.net/DanielGarfado/hol07-en) HOW TO: Calculate Km and Vmax with Excel Summary. This step by step article explains how to calculate Km and Vmax using Microsoft Excel. Solver add-in is available as...
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- April 24, 2015. Yamaha V-Max and VMAX - Wikipedia, the free encyclopedia (http://en.wikipedia.org/wiki/Yamaha_V-Max_and_VMAX) you plot v (Y-axis) against v/[S] (X-axis, [S] is substrate conc; intersection of linear graph at v = 0 with X-axis is Vmax/Km; -Km is slope
- April 25, 2015. Vmax - Biology-Online Dictionary - Life Science Reference... (http://www.biology-online.org/dictionary/Vmax) You need to know the 3 parts of the graph: a) initially linear since many free enzymes available at the lower concentration of substrate. b) rate begins to...
- May 13, 2015. How to calculate vmax lineweaver | eHow UK (http://www.ehow.co.uk/how_7714561_calculate-vmax-li neweaver.html) Estimate Km and Vmax from the new graph. Km stays the... Leggsase is likely to be a single-unit enzyme because the graph is typical of a single-unit and does not...
- May 14, 2015. Graph of Competitive and Noncompetitive Enzyme Inhibition (http://faculty.une.edu/com/courses/bionut/distbio/o bj-512/Chap9-enzymeinhibition.html) How to calculate vmax lineweaver. Vmax represents the rate at which an enzyme is completely saturated with substrate... (1/V) to obtain a straight-line graph.
- May 15, 2015. Enzyme Kinetics - MP & Associates - The most complete... (http://www.mpassociates.gr/software/catalog/sci/enz ymekinetics.html) Answer to a) A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (v0) at different substrate conce...
- May 16, 2015. How do you calculate Vmax from lineweaver burk plot... (https://uk.answers.yahoo.com/question/index?qid=200 91224063842AAgJmFu) BioChemistryFilter: How do I read the vMax and Km off of a Lineweaver-burk plot?
- May 17, 2015. How to find Vmax and Km using limeweaver burk method... (https://answers.yahoo.com/question/index?qid=200903 29180531AArDhN3) A graph of the double-reciprocal equation is also called a Lineweaver-Burk, 1/Vo vs 1/[S]. The y-intercept is 1/Vmax; the x-intercept is -1/KM; and the slope is KM/Vmax.
- May 18, 2015. How do you calculate Vmax from lineweaver burk plot... (https://answers.yahoo.com/question/index?qid=200912 24063842AAgJmFu) The effect of substrate concentration on enzyme activity. Skip the theory and go straight to: How to determine Km and Vmax. A simple chemical reaction with a single...
- May 19, 2015. Measuring KM and Vmax - Biology Hypertextbook (http://www.sciencegateway.org/resources/biologytext /eb/kinetics/MandM4.html) Equation: Michaelis-Menten model; Equation: Determine kcat; Equation:... (Smin is the smallest value of [substrate] you want to include on the graph) and Y=(1/Vmax)...
- May 20, 2015. Measuring KM and Vmax - Campbell University (http://web.campbell.edu/faculty/nemecz/323_lect/enz ymes/Measuring%20KM%20and%20Vmax%202.html) 3. Into row 2 enter X=1/Smin (Smin is the smallest value of [substrate] you want to include on the graph) and Y=(1/Vmax)(1.0 + KM/Smin).
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- February 7, 2016. How to graph lineweaver-burk plot on excel 2011 (http://enlptns.ga/mB) The graph should look like a rectangular hyperbola where higher concentrations of substrate... How to Calculate KCAT and VMAX. How to Plot a Michaelis-Menten Curve.
- February 8, 2016. How to determine vmax from a graph - 7k1os.ns0.it (http://7k1os.ns0.it/uCz) Author Topic: Enzyme Kinetics. Getting Km and Vmax from 4 graphs. (Read 4488 times)
- February 9, 2016. Exploratory EK Help - SigmaPlot (http://www.sigmaplot.com/products/sigmaplot/Explora tory_Enzyme_Kinectics_Help.pdf) This means that when you plot the graph of 1/V (on the y-axis) against 1/[S] (on the x-axis)... How do you calculate Vmax from lineweaver burk plot?
- February 10, 2016. VMAX function - MedCalc (https://www.medcalc.org/manual/VMAX_function.php) Answer to a) A graph of the Michaelis-Menten equation is a plot of a reaction's initial velocity (v0) at different substrate conce...
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- February 12, 2016. Intelligent BioProcessing Systems (http://il-biosystems.de/fileadmin/Produkt-PDFs/210_ FilterTec_Vmax_Filter_Determinations.pdf) You need to know the 3 parts of the graph... B.7.4 Determine Vmax and Michaelis constant (Km) by graphical means... How to calculate Vmax and Km from...
- February 13, 2016. Intelligent BioProcessing Systems (http://il-biosystems.de/fileadmin/Produkt-PDFs/210_ FilterTec_Vmax_Filter_Determinations.pdf) Michaelis-Menten Equation - Interactive Graph. When studying biochemical and physiological processes, it is often necessary to measure the rate at which a given...
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- February 15, 2016. Structural Biochemistry/Enzyme/Michaelis and Menten... (https://en.wikibooks.org/wiki/Structural_Biochemist ry/Enzyme/Michaelis_and_Menten_Equation) 3. Into row 2 enter X=1/Smin (Smin is the smallest value of [substrate] you want to include on the graph) and Y=(1/Vmax)(1.0 + KM/Smin).
- September 5, 2016. Results | VMAX200 (http://www.vmax200.com/results/) Vmax corresponds to the inverse o the y-intercept while Km is the product of the slope and the Vmax. From the Michaelis-Menten plot, you can obtain the equation of...
- September 6, 2016. VMAX Technical & Applications Charts (http://www.vmaxtanks.com/Technical-Data_ep_41.html) How do I use Michaelis-Menten and Lineweaver-Burk plots to determine Km and Vmax? Vmax is the maximum rate of an enzyme catalysed reaction i.e. when the enzyme is...
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- September 9, 2016. Michaelis-Menten Equation - Interactive Graph - PhysiologyWeb (http://www.physiologyweb.com/calculators/michaelis_ menten_equation_interactive_graph.html) A tutorial of linear regression for estimating Vmax and Km values with Excel By Andreas Jansson. In the laboratory you should have measured the velocity for a number...
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- February 15, 2017. Lab 3: Enzyme Kinetics - York College of Pennsylvania (http://faculty.ycp.edu/~jthompso/Biochemistry%20F20 09/labs/enzyme%20kinetics%202009.pdf) Vmax graph - [CLOSED] ON GRAPH SET LOOKGRAPH VLINE2 SET VAUTO OFF VMIN 100 VMAX... Jump-start your weight loss with us. Our tasty nutrition plans, healthy diet ideas...
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- February 17, 2017. How do you get the Vmax and Km from Michaelis-Menten graph... (https://socratic.org/questions/how-do-you-get-the-v max-and-km-from-michaelis-menten-graph) Graph is of concentration vs time d[ES]/dt is about 0... = Vmax/[E]t The number of reactions the active site catalyses per unit of time (turnover number)
- February 18, 2017. Enzyme Kinetics and Catalysis - Biol 230 Master - Confluence (https://wikispaces.psu.edu/display/230/Enzyme+Kinet ics+and+Catalysis) Iam measuring the Dapoxetine of substrate in a enzymatic reaction to find out the kinetic parameters Km and Vmax... I attached a graph from one of the papers...
- February 19, 2017. Basics of enzyme kinetics graphs (article) | Khan Academy (https://www.khanacademy.org/science/biology/energy- and-enzymes/enzyme-regulation/a/basics-of-enzyme-ki netics-graphs) You need to know the 3 parts of the graph: a)... B.7.4 Determine Vmax and Michaelis constant (Km) by graphical means and... Finding Vmax from the...
- February 20, 2017. Excel 2007 Tutorial | Cartesian Coordinate System (https://www.scribd.com/doc/44604727/Excel-2007-Tuto rial) How to read enzyme kinetics graphs (and how they're made). Km and Vmax. Competitive and noncompetitive inhibitors.
- February 21, 2017. 1998 Yamaha Vmax 1200 in Massachusetts, United States... (https://motorcyclegraph.com/ma/yamaha/vmax-1200/199 8) The quantities KM and Vmax are experimentally determined and different for each enzyme. Once you have an assay for enzyme activity, you can determine these parameters.
- February 22, 2017. VMAX Technical & Applications Charts (https://www.vmaxtanks.com/Technical-Data_ep_41.html) Michaelis-Menten Equation - Interactive Graph. When studying biochemical and physiological processes, it is often necessary to measure the rate at which a given...
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- August 12, 2017. 20. Two inhibitors of enzyme X were studied. Describe what... (https://www.coursehero.com/tutors-problems/Biochemi stry/10803080-Biochemistry-Final-Exam-Prep-Can-some one-help-me-with-these-Please-e/) Enzyme Technology Determination of V max and K m. It is important to have as thorough knowledge as is possible of the performance characteristics of enzymes, if they...
- August 13, 2017. How to Calculate KCAT and VMAX | Sciencing (http://sciencing.com/calculate-kcat-vmax-7866502.ht ml) Answer to 20. Two inhibitors of enzyme X were studied. Describe what type of inhibitor each is and how Km and Vmax are affected by the inhibitor. Explain why
- August 14, 2017. GraphPad - FAQ 1749 - Prism 3 Lineweaver-Burk plots (http://www.graphpad.com/support/faqid/1749/) Obtain rates of conversion for increasing concentrations of a substrate in a constant concentration of enzyme. Divide one by the rate of conversion for each data...
- January 1, 2018. How to calculate the km and Vmax values of an enzyme when I... (https://www.researchgate.net/post/How_to_calculate_ the_km_and_Vmax_values_of_an_enzyme_when_I_have_sub strate_product_inhibition) This Site Might Help You. RE: How do you calculate Vmax from lineweaver burk plot? I did this exercise on enzyme kinetics a few weeks ago and I can't...
- January 2, 2018. Enzyme Kinetics - UW-Madison Chemistry (http://www.chem.wisc.edu/deptfiles/genchem/netorial /modules/biomolecules/modules/enzymes/enzyme4.htm) How to calculate the km and Vmax values of an enzyme when I have substrate/product inhibition?
- January 3, 2018. Enzyme kinetics - Wikipedia (https://en.wikipedia.org/wiki/Enzyme_kinetics) In a mathematical description of enzyme action developed by Leonor Michaelis and Maud Menten in 1913... if the graph is extended along the x-axis...
- January 4, 2018. Enzyme Kinetics: Velocity - Purdue University (https://www.chem.purdue.edu/courses/chm333/Spring%2 02013/Lectures/Spring%202013%20Lecture%2015.pdf) Enzyme Kinetics and Catalysis... This graph illustrates the distribution of molecules of one reactant across a range of kinetic... Vmax is the maximal velocity...
- January 5, 2018. How to Plot a Michaelis-Menten Curve | Sciencing (https://sciencing.com/plot-michaelismenten-curve-10 044510.html) Enzyme kinetics is the study of the chemical... shown as the intercept on the y-axis of the graph... On the other hand, the Vmax will decrease relative to...
- January 6, 2018. How do I calculate a Michaelis menten curve from UV-VIS... (https://www.researchgate.net/post/How_do_I_calculat e_a_Michaelis_menten_curve_from_UV-VIS_spectrophoto meter_data) Enzyme Kinetics: Velocity... max can then be easily determined from the graph. - How can we do this: We take the reciprocal of both sides of the Michaelis-Menten...
- January 7, 2018. GraphPad Curve Fitting Guide (http://www.graphpad.com/guides/prism/7/curve-fittin g/index.htm?reg_michaelis_menten_enzyme.htm) Km and Vmax values are usually listed with the substrate as well in terms of millimoles and sec-1 when... Plotting the Michaelis-Menten Curve. Using graph paper...
- January 8, 2018. Determining Km and Vmax - Science and Plants for Schools (http://www.saps.org.uk/saps-associates/browse-q-and -a/162-how-do-i-use-michaelis-menten-and-lineweaver -burk-plots-to-determine-km-and-vmax) Michaelis-Menten and Lineweaver-Burk Plots... from the baseline-corrected Michaelis-Menten graph. The units for Vmax are the units that you used when entering Y...
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- February 4, 2018. Vmax: Blog by kaaz (http://kaaz.cocolog-nifty.com/blog/vmax/index.html) A visual approach to understanding resolution based on MTF (modulation transfer function) curves in film and lenses.
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- February 6, 2018. GraphPad Curve Fitting Guide (http://www.graphpad.com/guides/prism/7/curve-fittin g/index.htm?reg_kcat.htm) Hi I have some questions about substrate concentration and enzyme activity. In my lab, we were task to find the absorbance of 0.3mM, 1.5mM, 2.1mM, 2.7mM and 3.0mM of...
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